Transplant Biosupply
​LIFECODES is a world-class leader in transplant diagnostics providing a broad portfolio of molecular and antibody-based assays using ELISA and xMAP® technology to evaluate HLA compatibility between donors and recipients.
Laboratories all over the world use LIFECODES HLA serology and molecular typing assays as part of determining the best path forward for a transplant recipient. The more compatible the donor-recipient match, the less likely for organ or stem cell rejection.

ANTIBODY DETECTION PRODUCTS

SCREENING AND IDENTIFICATION LIFECODES

LifeScreen Deluxe contains seven different Class I microspheres and five Class II microspheres. Sensitivity is enhanced using microspheres enriched with CREG.
The identification panels Class I and II offer the identification of antibodies and PRA information (reactive antibodies on the panel).
The vacuum collector washing technique reduces the total time of active involvement, the variations caused by the operator and the contamination.

LIFECODES LSA ANCIENT UNIQUE

The LSA (LIFECODES Unique Antigen) kit is designed to identify allele-level antigens. It also allows the identification of those characteristics specimens that are masked in high PRA sera tested on conventional identification panels.
The Class I panel comprises over 90 different recombinant antigens HLA-A, -B and -Cw.
The Class II panel comprises over 60 recombinant HLA-DRB, -DQB and -DPB antigens.
It contains both common and less common antigens.
Each DQ and DP antigen contains different combinations of common alpha and beta chains, facilitating the identification of chain-specific antibodies.

LIFECODES LSA MIC

The LSA-MIC kit is designed to detect antibodies that act against MICA antigens. Publications suggest that anti-MICA antibodies can play a role in the rejection of renal allografts.
Most of the identified MICA antigens are represented on the panel to provide extensive coverage of MICA amino acid positions.
Most immunogenic epitopes are present in several antigens.
The solid phase test using Luminex's xMAP technology ensures the sensitivity of flow cytometry.

LIFECODES DONOR SPECIFIC ANTIBODIES

Donor Specific Antibodies (DSAs) have been shown to be involved in both acute and chronic rejection of transplanted allografts. Immucor offers solutions for DSA screening in transplant samples.
LIFECODES DSA Detection of donor specific antibodies.
DSA facilitates the detection of the presence of antibodies that act against the donor's HLA antigens. It is ideal for pre- and post-testing post-transplant.
 Donor lymphocytes are first isolated and lysed. The lysed material is then incubated with Class I and Class II specific microspheres. Donor HLA antigens bind to specific microspheres via monoclonal capture antibodies. Subsequently, the microspheres are incubated with serum and any HLA antibodies that act against the donor are detected with the Luminex analyzer after adding a conjugate PE-labeled human anti-IgG.
Detection of donor-specific anti-HLA IgG antibodies to Class I and / or Class II antigens in a single procedure.
Freezing of the lysed material from the donor for future tests.
No problems with cell viability.
The values ​​reflect the displacements of the compatibility channel in the flow.
Perform between 1 and 91 tests at the same time.


HLA GENOTYPE

LIFECODES TYPIFICATION HLA SSO - RAPID (launched in March 2014)

HLA LIFECODES typing kits - Rapidly uses the SSO methodology to identify the HLA alleles present in a specific sample of the locus amplified by PCR.
LIFECODES Taq polymerase is included in each kit.
Results in less than two hours.
No centrifugation or washing - thus reducing the total time of active involvement.
The mixture includes primers, dNTP and PCR buffer - eliminating additional steps and consumables.

NEW TYPING KITS LIFECODES

The new HLA LIFECODES typing kits use the SSO methodology to identify the HLA alleles present in an amplified specific locus sample by PCR. LIFECODES Taq polymerase is included in each kit.
The new LIFECODES Taq polymerase is validated and included in the kit.
 Improved probe performance - signal strength and signal-to-noise ratio are improved.
Improved tolerance to PCR inhibitors.
The mixture includes primers, dNTP and PCR buffer - eliminating additional steps and consumables.
Homogeneous test, WASHING MACHINE for the hybridization procedure. Thus, the need for centrifugation is eliminated and the risk of pipetting errors is reduced; save both time and lab supplies.
The analysis software automatically imports the data from the Luminex instrument.


HLA-Ready Plate systems for serological typing of HLA antigens. Ready-to-use plates are available for determining HLA antigens class I and class II in different dimensions.
All Ready Plate kits conform to EC Directive DDIV 98/79 / EC, are CE marked and are manufactured according to accredited quality systems DIN EN ISO13485: 2012.
Integrated determination of null alleles A * 24: 09N, B * 51: 11N, C * 04: 09N, DRB4 * 01: 03N, DRB5 * 01: 08N.
Integrated negative control.
Short analysis due to allele specific amplification.
Regular updating of the IMGT HLA database.
Simplified interpretation by allele frequency (CWD).
Mixtures of previously divided and dried primers.
Kits with color codes.
Reducing errors by modifying internal control amplification products.
Flexibility due to the combination of PCR plates.
A PCR program for all systems.
Identical workflow with protocol sheets adapted for all kits.
Evaluation with SCORE software.
All Ready Gene kits conform to the EC Directive DDIV 98/79 / EC, are CE marked and are manufactured according to DIN EN ISO13485: 2012 accredited quality systems.
FluoGene is a unique molecular typing method for HLA-, RBC- and HPA, combining all the advantages of SSP-PCR with the speed of fluorescence signal detection. The analysis is based on a specially modified TaqMan® probe system detected with the FluoVista Analyzer.
ADVANTAGES

PCR-SSP method without gel electrophoresis in 90 minutes.
No hybridization and washing.
Display of typing results immediately after post-reading.
No risk of post-PCR contamination.
Low DNA consumption. Easy to use software solution, fully automatic calculation of results.
Same system for HLA-, RBC- and HPA.
All FluoGene kits comply with EC Directive DDIV 98/79 / EC, are CE marked and are manufactured according to DIN accredited quality systems EN ISO13485: 2012.