FluoGene is a unique method for molecular HLA-, RBC- and HPA typing combining all advantages of the SSP – PCR with the speed of endpoint fluorescence detection. The analysis is based on specifically modified TaqMan® probe system by the inno-train Diagnostik GmbH.
FluoGene is a unique method for molecular HLA-, RBC- and HPA typing combining all advantages of the SSP – PCR with the speed of endpoint fluorescence detection. The analysis is based on specifically modified TaqMan® probe system by the inno-train Diagnostik GmbH. Detection with integrated evaluation is realized in the semi-automated FluoVista version by the FluoGene evaluation software. By the use of a barcode scanner plate-specific data can be easily read from the FluoGene tray or sample-ID can be scanned and be automatically transferred into the FluoGene Software. The fully- automated FluoStation version with an integrated pipetting unit is already equipped with a barcode scanner. FluoGene is a self-contained system comprising the ready-to-use Kits, the detection device and the software solution displaying the typing results immediately after the evaluation read. For the PCR set-up simply add your diluted sample DNA to the ready-to-use FluoMix and dispense 15 μl per well over the PCR plate. This manual step is performed automatically by the FluoStation version. Afterwards the FluoGene plates are sealed with an adhesive optical foil. The subsequent pre- and post- reads are generated in a closed environment. Therefore any risk of a post-PCR contamination gets eliminated. The FluoGene Software supplies the immediate display of typing results, as well as a fully-automated data management.
ADVANTAGES OF THE FLUOGENE SYSTEM
- PCR-SSP method without gel electrophoresis within 90 minutes
- No hybridization and washing steps
- Display of typing results immediately after the post-read
- No risk of post-PCR contaminations
- Low DNA consumption (app. 5-10ng per PCR reaction)
- User-friendly software solution, fully-automated calculation of results
- Same system for HLA-, RBC- and HPA